The University of Sheffield has identified that knocking down the level of expression of a specific endogenous protein, ubiquitous in mammalian cells, results in a significant increase in recombinant protein production. This observation provides a novel route to increase recombinant protein production by mammalian cells either in rapid, transient format or potentially for continuous production from stably engineered cells.
Significant challenges remain in the production of proteins from mammalian cell expression systems and the ability to increase levels of recombinant protein production by mammalian cells would be an important advance for the biotechnology industry. The University of Sheffield has identified that knocking down the level of expression of a specific endogenous protein, ubiquitous in mammalian cells, results in a significant increase in recombinant protein production. This observation provides a novel route to increase recombinant protein production by mammalian cells either in rapid, transient format or potentially for continuous production from stably engineered cells.
Knock-down of this single host cell protein results in a 4-5 fold increase in recombinant protein production during transient transfection of mammalian cells and the increase in expresion is observed for both secreted and non-secreted proteins. It has been shown that the effect is independent of transfection reagent and target protein.
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